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KMID : 0613820030130040421
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Journal of Life Science
2003 Volume.13 No. 4 p.421 ~ p.428
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Characteristics of Heavy Metal Biosorption by Enterobacter intermedious KH410
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Kim Young-Hee
Jeong Young-kee
Kim Kwang-Hyeon
Kim Byung-Woo
Chung Kyung-Tae
Kim Byung-Suk
Park Ji-Won
Lee Dong-Jun
Shin Hyun-Chul
Lee Seung-Gee
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Abstract
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A natural habitat bacterium, Enterobacter intermedious KH410 was isolated from freshwater plant root and identified. Adsorption of heavy metals such as lead, cadmium, and copper by this strain was examined. The minimal inhibitory concentrations(MIC) for each metal were 1.78 mM for lead, 0.17 mM for cadmium and 1.39 mM for copper, respectively. Maximum production of dried cell was 2.56 g/ℓ in LB medium containing 0.5% NaCl, 1% yeast extract and 1% of lactose. Optimal conditions for adsorption were 0.6 dry g-biomass, at pH 4.0 and the temperature of 20℃. Adsorption equilibrium reached maximum after 30 min in 400 ㎎/ℓ metal solution. The adsorption capacity (K) of copper was 1.5 times higher than that of cadmium and lead was 1.1 times higher than that of cadmium. From the results obtained in this study, Freundlich adsorption model was applicable for all metals. Adsorption strength (l/n) of heavy metal ions were in the order of cadmium>copper>lead. The adsorption of dried cell for lead, cadmium, and copper was 56.2, 58.0, 55.8 ㎎/g-biomass, respectively. Pretreatment to increase ion strength was the most effective with 0.1 M NaOH whereas slight difference was found both KOH and CaCl₂ upon same concentration. Effective desorption was induced by 0.1 M EDTA for lead and 0.1 M HNO₃ for cadmium and copper.
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KEYWORD
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adsorption, Cd, Cu, Pb, Enterobacter intermedious KH410
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